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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Calcium. 2009 Jun 11;46(1):73–84. doi: 10.1016/j.ceca.2009.05.003

Fig. 2.

Fig. 2

Adenovirus vector expressing TC4sh1 produced a specific TRPC4 mRNA knockdown in PHM1-41 cells. (A) PHM1-41 infection with adenovirus expressing TC4sh1 induced specific TRPC4 mRNA knockdown compared to effects of empty vector (E) or scrambled shRNA (shx1). Also shown are the TRPC mRNA levels in PHM1-41 cells not treated with adenovirus (UN). mRNA levels of other TRPCs are unchanged by exposure to any of these viral constructs. mRNA was normalized to GUS in the same sample and expressed relative to the value in cells exposed to empty vector. In all cases, data represent the mean ± S.E.M. (n=3); significant differences between groups (p<0.05), as analyzed by one-way ANOVA and Tukey's test, are indicated by different lowercase letters. (B) RT-qPCR products for TRPC1, TRPC4, TRPC6, and GUS (obtained from 100 ng RNA) as well as for TRPC3, TRPC5 and TRPC7 (obtained from 1 μg RNA) were run in 3% agarose gels. In all cases, single bands of the expected size were observed. No bands are observed in samples lacking reverse transcriptase (RT) in the reaction.