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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cell Calcium. 2009 Jun 11;46(1):73–84. doi: 10.1016/j.ceca.2009.05.003

Fig 3.

Fig 3

Adenovirus vector expressing TC4sh1 produced a specific TRPC4 protein knockdown in PHM1-41 cells. (A). whole cell extracts and (B) purified membrane extracts from uninfected (UN) cells and cells exposed to empty adenovirus (E), virus expressing a scrambled shRNA sequence (shx1), or virus expressing TC4sh1 for > 72 h. PLCB3 was used as normalization control. (C) TRPC4 antibody (Ab) specificity is shown by the absence of 92 kDa band when the antibody was preabsorbed with TRPC4 antigenic peptide. (D) Antibody preabsorption usingTRPC1 antigenic peptide specifically blocks 116 KDa TRPC1 band detection by the TRPC1 antibody (Ab). (E) Expression of the TRPC4-HA tag protein overexpressed (TRPC4 OE) in COSM6 cells and suppression of this expression by cotransfection with the TC4sh1 construct.