Aliquots of 5 (lanes 3,5 starting from the left lane) or 2.5 μm (lanes 2,4) of EMD or amelogenin were applied to the gel. Standard molecular weight markers were run in lanes 1 and 6. The gel was stained with Cosmassie Brilliant Blue R-250. Note that EMD contains a mixture of proteins, the majority of which are relatively low molecular weight, <30 kDa, with the exception of one prominent band in the area of 52.2 kDa. By contrast, the major band of the amelogenin preparation occurs at a molecular weight of 28.9 kDa. Although a few weak bands present at higher molecular weights greater than 50 kDa exist, there are no protein bands present at molecular weights below 28.9 kDa.