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. Author manuscript; available in PMC: 2010 May 10.
Published in final edited form as: J Periodontol. 2009 Apr;80(4):648–656. doi: 10.1902/jop.2009.070420

Figure 2. SDS-PAGE of Amelogenin and Enamel Matrix Derivative.

Figure 2

Aliquots of 5 (lanes 3,5 starting from the left lane) or 2.5 μm (lanes 2,4) of EMD or amelogenin were applied to the gel. Standard molecular weight markers were run in lanes 1 and 6. The gel was stained with Cosmassie Brilliant Blue R-250. Note that EMD contains a mixture of proteins, the majority of which are relatively low molecular weight, <30 kDa, with the exception of one prominent band in the area of 52.2 kDa. By contrast, the major band of the amelogenin preparation occurs at a molecular weight of 28.9 kDa. Although a few weak bands present at higher molecular weights greater than 50 kDa exist, there are no protein bands present at molecular weights below 28.9 kDa.