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. Author manuscript; available in PMC: 2010 May 10.
Published in final edited form as: J Periodontol. 2009 Apr;80(4):648–656. doi: 10.1902/jop.2009.070420

Figure 3.

Figure 3

Figure 3a. Alkaline Phosphatase Activity at Day 5 of EMD Fractions on C2C12 Cells. When the alkaline phosphatase activity of each fraction was determined and superimposed on the EMD protein separation profile, the strongest activity occurred in fractions 12–17, which does not correspond to any of the major EMD protein peaks.

Figure 3b. Noggin Inhibition of Day 5 Alkaline Phosphatase Activity of EMD Fractions on C2C12 Cells. Prior to application to the cells, fractions were preincubated for 1 hour with 100ng/ml noggin, a decoy receptor known to bind to BMP 2, 4, and 7, and block their activity. Alkaline phosphatase activity was decreased, especially in the area where the highest activity occurred prior to noggin treatment, suggesting that the osteoinductive activity associated with EMD is not related to amelogenin, but more likely with a member of the BMP family of proteins.