Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 May 10;5(5):e10430. doi: 10.1371/journal.pone.0010430

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Dubielecka et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

A. Live confocal observations of washed cells indicate that Alexa Fluor 647 accumulates in large vesicular structures enriched in cell extensions of LNCaP cells. Representative merged image from DIC and Alexa Fluor 647 channel is presented. Boxed area is presented below. Merge, indicates merged image from DIC and Alexa Fluor 647 channel; Alexa Fluor 647, indicates Alexa Fluor 647 channel; DIC+, indicates DIC channel with enhanced contrast to better visualize vesicular-like structures. Bar, 10 µm. B. Live confocal image of LNCaP cells immersed in cell culture medium containing Alexa Fluor 647. Observations were carried out without removal of Alexa Fluor 647. Representative images from merged DIC and Alexa Fluor 647 channels (lower left panel) and DIC (upper left panel) are presented. Boxed area is presented as enlarged images in right panels: Lower right panel indicates merged image from DIC and Alexa Fluor 647 channels; middle right panel indicates DIC channel; upper right panel indicates DIC with enhanced contrast. Bar, 10 µm. C. Flow cytometric analysis indicates time-dependent uptake of Alexa Fluor 647 in LNCaP cells. Cellular accumulation of the fluorescent marker was determined by flow cytometry at the indicated time points following addition of Alexa Fluor 647 as detailed in Methods (n = 4, error bars ± s.e.m.). D. Quantification of intracellular accumulation of Alexa Fluor 647 following a 2-hour incubation with the dye. LNCaP, LNCaP cell line; Mock, mock transfected cell line; Iso-2.1 and Iso-2.2, isoform 2 expressing cell lines; Iso-3.1 and Iso-3.2, isoform 3 expressing cell lines. For each cell line measurements were performed on triplicate samples; error bars represent ± s.e.m.; Mock, n = 4; LNCaP, Iso-2.1, Iso-2.2, Iso-3.1, and Iso-3.2, n = 7.