Skip to main content
. 2010 May 10;5(5):e10430. doi: 10.1371/journal.pone.0010430

Figure 5. Mapping of Rac1 interactions with recombinant Abi1.

Figure 5

A. Schematic representation of Abi1 truncated mutants used for mapping and Rac1 structure. A total of eight Abi1 constructs were used, each represents a major domain or region in the Abi1 protein (Fig. 1A). Rac1 contains three major GTP binding sites (GTPbs), two switch regions (SI and SII) and GEF/GAP/GDI binding region. Three mutations are depicted: N-terminal mutations T17N (dominant negative), V12G and Q61L (constitutively active). B. Binding of Rac1 to Abi1 truncated products. Inactive Rac1 binds to the N-terminal truncated mutant encompassing N-186 residues, and active Rac1 mutant shows exon 10 dependence in binding. Active Rac1Q61L binds weakly to this region. Active Rac1Q61L also shows exon 10 dependent binding to the 303-C region. Observations are summarized in Table below.