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. 2010 May 10;5(5):e10558. doi: 10.1371/journal.pone.0010558

Figure 4. CD4-blockade prevents Th17 polarization.

Figure 4

(A) Sorted TCR-transgenic cells were stimulated in vitro with peptide-loaded DCs under culture conditions known to preferentially polarize Th17 cells, with the addition of recombinant TGF-β, IL-6, IL-1β, and anti-IFN-γ. After 5 days of culture we observed a significant reduction of IL-17+ cells in the presence of anti-CD4 (n = 6, P<0.05). In contrast, anti-CD4 addition led to an increased frequency of Foxp3+ T cells (n = 6, P<0.05). (B) Representative dot plots from the two different culture conditions. An independent experiment was performed with a peptide dose of 0.3 µM with similar results.