Table 1.
Gel spot | Protein name (abbreviated) | MOWSE scorea | Peptide countb | Protein score CI%c | Accession no.d | Protein MW (Da) | Protein pI | Summary of function |
---|---|---|---|---|---|---|---|---|
N1 | Sorcin (P30626) | 133 | 5 | 100 | SORCN CRILOe | 22017.4 | 5.32 | Calcium-binding protein |
N4 | Golgi autoantigen | 90 | 24 | 99.951 | GI: 808869f | 372014.8 | 4.96 | May participate in forming intercisternal cross bridges of the Golgi complex |
N7 | pyruvate dehydrogenase | 69 | 7 | 99.596 | ODPB_RATe | 39,336 | 5.94 | Glycolysis |
N8 | mu-crystallin | 135 | 4 | 100 | IPI00214448 | 33704.1 | 5.34 | Ammonia elimination |
N9 | pyrophosphatase | 67 | 7 | 99.283 | IPI00371957 | 33206.3 | 5.26 | Signal transduction |
N12 | creatinine kinase | 543 | 20 | 100 | IPI00470288 | 42983.4 | 5.39 | Energy transduction |
N13 | Sec 1413 protein | 131 | 6 | 100 | IPI00208939 | 46453.5 | 5.64 | Hydrophobic ligand binding |
N19 | creatinine kinase | 299 | 10 | 100 | IPI00470288 | 42983.4 | 5.39 | Energy transduction |
N23 | actin | 259 | 10 | 100 | IPI00369618 | 59162.6 | 5.67 | Structural |
N24 | eukaryotic translation initiation factor 4A1 | 129 | 14 | 100 | IPI00369618 | 46352.6 | 5.32 | Protein synthesis initiation |
N25 | beta actin | 447 | 10 | 100 | GI: 62897625f | 41737.8 | 5.37 | Structural |
N26 | actin, cytoplasmic | 420 | 15 | 100 | IPI00767505 | 42108.9 | 5.31 | Structural |
N27 | actin | 753 | 17 | 100 | IPI00194087 | 42,334 | 5.23 | Structural |
N29 | actin, cytoplasmic | 198 | 6 | 100 | IPI00765011 | 59162.6 | 5.67 | Structural |
N32 | actin, cytoplasmic | 167 | 4 | 100 | IPI00765011 | 59162.6 | 5.67 | Structural |
N33 | actin, cytoplasmic | 186 | 5 | 100 | IPI00765011 | 59162.6 | 5.67 | Structural |
N41 | ATP synthase | 919 | 21 | 100 | IPI00551812 | 56318.5 | 5.19 | ATP catabolism |
N43 | ATP synthase | 160 | 17 | 100 | IPI00551812 | 56318.5 | 5.19 | ATP catabolism |
N47 | heat shock protein 1A (chaperonin) (Hsp 60) | 220 | 27 | 100 | IPI00339148 | 61088.4 | 5.91 | Stress-induced chaperone; protein folding |
N51 | heat shock 70 kDa 9 A (predicted) | 419 | 9 | 100 | IPI00363265 | 74,097 | 5.97 | Protein folding; protein export; binds ATP, nucleotides, and proteins |
N53 | heat shock 70 kDa 9 A (predicted) | 64 | 5 | 98.428 | IPI00858386 | 74098.9 | 5.97 | Protein folding; protein export; binds ATP, nucleotides, and proteins |
N55 | Vomeromodulin | 87 | 8 | 99.992 | IPI00209782 | 79527.3 | 5.92 | Putative pheromone and odorant transporter |
N56 | heat shock 70 kDa, protein 5 | 621 | 15 | 100 | IPI00206624 | 72473.5 | 5.07 | Assembly of multimeric protein complexes |
N57 | heat shock 70 kDa, protein 5 | 896 | 27 | 100 | IPI00206624 | 72473.5 | 5.07 | Assembly of multimeric protein complexes |
N61 | tyrosine 3-mono-oxygenase Tryptophan 5 | 243 | 12 | 100 | IPI00230835 | 28,456 | 4.8 | Adapter protein; signal transduction |
N63 | tubulin | 485 | 12 | 100 | IPI00189795 | 50787.9 | 4.94 | Structural |
N64 | tubulin | 174 | 16 | 100 | IPI00339167 | 50119.6 | 4.94 | Structural |
N65 | tubulin | 148 | 14 | 100 | IPI00339167 | 50119.6 | 4.94 | Structural |
N66 | tubulin | 258 | 23 | 100 | IPI00400573 | 49,769 | 4.79 | Structural |
N67 | tubulin | 464 | 24 | 100 | IPI00197579 | 49,639 | 4.78 | Structural |
N68 | protein disulfide isomerase | 272 | 17 | 100 | IPI00198887 | 56915.7 | 4.82 | Cell redox homeostasis |
N69 | protein disulfide isomerase | 139 | 15 | 100 | IPI00198887 | 56915.7 | 4.82 | Cell redox homeostasis |
N70 | protein disulfide isomerase | 227 | 25 | 100 | IPI00198887 | 56915.7 | 4.82 | Cell redox homeostasis |
N73 | ubiquinol-cytochrome c reductase core protein 1 | 162 | 16 | 100 | IPI00471577 | 57815.4 | 5.57 | Mitochondrial electron transport |
N74 | ubiquinol-cytochrome c reductase core protein 1 | 78 | 19 | 99.939 | IPI00471577 | 52815.4 | 5.57 | Mitochondrial electron transport |
N75 | chloride intracellular channel 1 | 212 | 14 | 100 | IPI00421995 | 26963.8 | 5.09 | Macrophage activation |
N76 | tubulin | 166 | 12 | 100 | IPI0018975 | 5103.6 | 4.94 | Structural |
N77 | tubulin | 126 | 19 | 100 | IPI00400573 | 49,769 | 4.79 | Structural |
The probability-based MOWSE score from MASCOT.
Peptides used for matching within the database.
Reflects the probability that this same match would be made in a different database.
Accession numbers are from the IPI rat database except where noted.
From the Swiss-Prot database searching mammals.
From the NCBI database searching mammals.