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. 2010 May 10;5(5):e10566. doi: 10.1371/journal.pone.0010566

Figure 1. Overview of the rapid method for isolating auto-antibody against tumor-associated antigen (TAA) using a scFv library.

Figure 1

The tumor-homing scFv-presenting phages were collected from tumors that were injected with a scFv library. The collected phages were infected to HB2151 for scFv secretion. The secreted scFvs from HB2151 were transferred to nitrocellulose membranes by colony lift. The membranes were incubated with tumor lysate followed by serum from a tumor-bearing mouse. The scFv-tumor protein complex was detected by auto-antibodies. The complex was digested into peptide by trypsin and analyzed using MALDI-TOF mass spectrometry for identification.