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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Exp Parasitol. 2010 Jan 28;125(3):222–229. doi: 10.1016/j.exppara.2010.01.014

Figure 1. Newly Differentiated ILTat 1.3 Procyclic T. brucei Contain GPI-PLCp Activity.

Figure 1

T. brucei ILTat 1.3 was suspended in a transformation medium (SDM-79) at of 4 × 106/ml and incubated at 27°C. At 24 h intervals, 2 × 107 parasites were harvested. (A), Total GPI-PLCp activity (Units/107 cells) was determined. Activity in cells at day 0 of differentiation was assigned a value of 100%. (B), Cell density during differentiation of ILTat 1. (C), Western blotting with anti-VSG117 (106/lane), anti-procyclin (6 × 106/lane), or anti-tubulin antibody (2 × 106/lane). PCF, established procyclic; Day #, refers to days post initiation of differentiation; TUB, tubulin.