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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Exp Parasitol. 2010 Jan 28;125(3):222–229. doi: 10.1016/j.exppara.2010.01.014

Figure 3. Differentiation, Cell Division, and Loss of GPI-PLCp in AnTat 1.1 T. brucei.

Figure 3

AnTat 1.1 T. brucei was resuspended in SM transformation medium (with 5 mM citrate and 5 mM cis-aconitate) at a density of 2 × 106/ml, and incubated at 27°C. At 12 h intervals, cells were counted, 2 × 107 parasites were harvested to be processed for GPI-PLCp activity or immunofluorescence assays (Materials and Methods). While in culture cells were counted and diluted to 105/ml when the density approached 107/ml. Cell density at any time point is calculated after correcting for the dilution factor. Relative GPI-PLC activity is plotted where amount of enzyme activity at Day 0 is assigned a value of 1000. (A), Detection of VSG and procyclin by immunofluorescence. (B), Replication of cells during and after transformation.