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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Exp Parasitol. 2010 Jan 28;125(3):222–229. doi: 10.1016/j.exppara.2010.01.014

Figure 5. Synthesis of GPI-PLCp after initiation of Differentiation.

Figure 5

T. brucei 427 bloodstream form was placed in a SDM-79 differentiation medium at 27°C. At specified intervals, 1.4 × 108 cells were labeled metabolically with [35S]methionine at 27°C. All samples were analyzed by SDS-PAGE and phosphorimaging. (A), affinity-adsorbed GPI-PLCp. (B), membrane protein profile (2 × 107 per lane). (C), quantitation of data presented in panel A.