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. Author manuscript; available in PMC: 2010 Dec 1.

Published in final edited form as: Cell Death Differ. 2010 Jan 8;17(6):984–993. doi: 10.1038/cdd.2009.199

FIG. 2 — (A) Histones were released during apoptotic internucleosomal degradation. Nuclei were isolated from control and dexamethasone treated cells and incubated with a lysis buffer containing 0.5% Triton X-100 at 4°C. Proteins contained in the lysis buffer were analyzed on SDS polyacrylamide gel electrophoresis and stained with 0.1% Coomassie Brilliant Blue.

(B) Thymocytes were incubated at 37°C for 1, 2, 3 and 4 hrs with 1 µM dexamethasone. Soluble (S) and insoluble (I) chromatin were isolated from nuclei. Histones were extracted from the chromatin and analyzed on SDS PAGE. left; Western blots were conducted with an anti-phosS14. C; untreated thymocytes incubated at 37°C. Proteins were stained with Ponceau-S. Right; Western blots were conducted with an acK8H4 antibody. U; untreated thymocytes on ice, C; control thymocytes, D; dexamethasone treated thymocytes at 37°C.

FIG. 2 — (A) Histones were released during apoptotic internucleosomal degradation. Nuclei were isolated from control and dexamethasone treated cells and incubated with a lysis buffer containing 0.5% Triton X-100 at 4°C. Proteins contained in the lysis buffer were analyzed on SDS polyacrylamide gel electrophoresis and stained with 0.1% Coomassie Brilliant Blue.

(B) Thymocytes were incubated at 37°C for 1, 2, 3 and 4 hrs with 1 µM dexamethasone. Soluble (S) and insoluble (I) chromatin were isolated from nuclei. Histones were extracted from the chromatin and analyzed on SDS PAGE. left; Western blots were conducted with an anti-phosS14. C; untreated thymocytes incubated at 37°C. Proteins were stained with Ponceau-S. Right; Western blots were conducted with an acK8H4 antibody. U; untreated thymocytes on ice, C; control thymocytes, D; dexamethasone treated thymocytes at 37°C.