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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2010 Jan 12;48(6):1060–1070. doi: 10.1016/j.yjmcc.2009.11.020

Figure 1. Expression of Netrin-1 receptors in the heart and netrin-1 stimulation of cardiac nitric oxide (NO•) production.

Figure 1

Whole mouse hearts (C57BL/6J) were homogenated for RNA and protein extraction. (A) Expression of DCC, neogenin, UNC5A -D, integrin β1, β4, α3, and α6 mRNA in the mouse heart was measured by RT-PCR and normalized to levels of GAPDH. In additional experiments, mouse hearts were freshly isolated and perfused with netrin-1 (100 ng/ml) on the Langendorff rig for 120 min. Hearts were then homogenated and incubated with the NO•-specific spin trap Fe2+(DETC)2 for 60 min prior to ESR analysis of NO• production. (B) Representative ESR spectra. (C) Grouped data from six independent experiments (Means±SEM, n=6), *p<0.01.