Figure 1. KChIP2 increases Kv4.2 and Kv4.3 expression.
Representative immunoblots of individual experiments showing neonatal rat myocytes express far less KChIP2 compared to adult myocytes(A); the expression of Kv4.2 and Kv4.3 is increased following KChIP2 overexpression in neonatal myocytes compared to β-gal (B). Kv4.3 induction shows two bands (66 and ~45/50 kDa). Protein expression was normalized to GAPDH. Bar graphs of fold change in the protein levels are shown in (C). (D) Representative RT-PCR and (E) quantification of mRNA expression of Kv4.2, Kv4.3 and Kv1.4 normalized to GAPDH mRNA (n=4). Data from 3–4 experiments.#, * P< 0.05 Ad.KChIP2 vs. Ad.β-gal.