Fig. 2.

Effect of p38MAPK inhibitor SB203580 on the phosphorylation status of p38MAPK and Akt and on the mRNA and protein levels of Hsp70 and MnSOD in V79 fibroblasts subjected to chronic heat stress. a phosphorylation status of p38MAPK (uppermost panel) and Akt (last but one panel from top) and protein expression of Hsp70 (third panel from top), Hsp72 (fourth panel from top), MnSOD (fifth panel from top). SOD enzyme activity is presented in the sixth panel from top. The last panel from top shows beta actin to indicate protein loading. b mRNA level of Hsp70 (uppermost panel), MnSOD (second panel from top), and beta-actin (last panel from top to indicate loading). The numbers on the top of the blots are indicative of the respective band densities when the control is normalized to 1 Details of the experimental procedure are given in the materials and methods section. Cells were either left untreated (Vctrl), treated with chronic heat stress (V4WkHS) or treated simultaneously with chronic heat shock and 5 µM SB203580 (V4WkHS + SB). The results are representative of two-three experiments