Fig. 2.

Gene expression analysis. Northern blot analysis of hsp70-1 (NCU09602.3), hsp70-2 (NCU08693.3), and hsp70-3 (NCU01499.3) transcripts using total RNA obtained from N. crassa mycelia. The wild-type strain was cultured for 15 h at 30°C in low- or high-Pi liquid medium (0.1 and 10 mM Pi, respectively) at pH 5.4 or 7.8. The effect of heat shock was assayed in mycelia incubated for 1 or 2 h at 45°C. The ethidium bromide-stained rRNA band is shown as a loading control. The β-tubulin gene was hybridized as additional loading control on the northern blot. Bars show fold expression determined from the intensity measured by densitometric analysis. Data are average values ± standard deviation (SD) obtained from two independent experiments