Fig. 5.
Gene expression analysis. Northern blot analysis of hsp70-1 (NCU09602.3), hsp70-2 (NCU08693.3), and hsp70-3 (NCU01499.3) transcripts using total RNA obtained from N. crassa mycelia. The wild-type (wt) and nuc-1RIP strains were cultured for 15 h at 30°C in a low-Pi liquid medium (0.1 mM Pi) at pH 5.4 or 7.8. The effect of heat shock was assayed in mycelia incubated for 1 or 2 h at 45°C. The ethidium bromide-stained rRNA band is a loading control. The β-tubulin gene acted as additional loading control for the northern blots. The bars show fold expression determined from the intensity measured by densitometric analysis. Data are average values ± standard deviation (SD) obtained from two independent experiments