Fig. 2.

Time course of proteasome inhibitor-induced HSP30, HSP70, and actin protein. Cells were maintained at 22°C or treated with lactacystin (10 μM; a), 10 μM MG132 (b), or 50 μM MG132 (c) for different time intervals ranging from 2 to 24 h in duration. Protein was transferred to nitrocellulose membranes from SDS-polyacrylamide gels and probed with anti-HSP30, anti-HSP70, or anti-actin polyclonal antibodies as described in “Materials and methods” section. In each of the panels, the data are representative of four different experiments