Fig. 5.

Cellular localization of lactacystin-induced HSP30 by LSCM. Cells were grown on glass coverslips and were maintained at 22°C (C) or treated with 5, 10, or 15 μM lactacystin for 15 h at 22°C. Actin and nuclei were stained directly with phalloidin conjugated to TRITC (red) and DAPI (blue), respectively. HSP30 was indirectly detected with an anti-HSP30 antibody and a secondary antibody conjugated to Alexa-488 (green). The white arrows indicate large circular cytoplasmic foci of HSP30 observed in some cells (bottom set of panels) treated with 15 μM lactacystin for 15 h. The 10-μm white scale bars are indicated at the bottom right section of each panel. These data are representative of five separate experiments