Fig. 4.

UPR targets CHOP and Herp are regulated differently during B-cell differentiation. a Cytosolic RNA from I.29 µ⁺ B cells stimulated with either LPS or Tg for the indicated periods of time were harvested, separated, and then probed for CHOP and Herp. 28S rRNA was probed as a loading control. b Cell extracts were prepared from cells treated as indicated and CHOP protein expression was determined by Western blotting. Hsc70 protein served as a control for loading. c Cytosolic RNA from WT and PERK null MEFs treated with Tg for the indicated periods of time were harvested, separated, and then probed for CHOP, Herp, and total XBP-1 (tXBP-1) transcript levels. Again, 28S rRNA was probed as a loading control