Fig. 8.

p58^IPK, but not GADD34, is induced during the differentiation of I.29 μ⁺ B cells into plasma cells. a I.29 μ⁺ B cells were treated with LPS or Tg for the indicated periods of time and RNA was isolated and prepared for Northern blotting. The membrane was probed for GADD34 and p58^IPK. 28S rRNA levels were also examined to control for loading. b Cell lysates were prepared from I.29 μ⁺ B cells that had been treated with LPS or from NIH3T3 fibroblasts treated with Tm for the indicated periods of time. Samples were examined on a 10% SDS-PAGE and transferred. The membrane was then blotted for p58^IPK protein and actin as a loading control