Fig. 9.

LPS pretreatment of I.29 μ⁺ B cells augment XBP-1(S) levels but inhibits CHOP induction by pharmacological ER stressors. I.29 μ⁺ B cells were pretreated with LPS for 0, 12, 24, or 36 h and then further cultured with Tm or Tg for 0, 3 or 6 h. Cell lysates were harvested for each experimental group and analyzed by SDS-PAGE. After transferring, the membranes were blotted for XBP-1(S) (left panel), CHOP (middle panel) or Hsc70 (right panel) as a control for loading