Fig. 2.

Expression and translocation of nucleolin in apoptotic HUVECs. a The mRNA levels of nucleolin were determined by RT-PCR. b The protein levels of nucleolin were determined by Western blot analysis. The results were expressed as mean ± SEM of relative values from three experiments in duplicates. **P < 0.01, statistically significant versus the control group (0 h). c The cells were treated with 0.5 mmol/L H₂O₂ for 1 and 3 h, respectively, and cytosolic (Cyto) and nucleolar (Nuc) extracts were separated by 12% SDS-PAGE and analyzed by immunoblotting with anti-C23, anti-PCNA, and anti-GAPDH