Table I.
Thermodynamic and Kinetic Parameters of Rop Variants
| M.R.E.a (deg cm2 dmol−1) | TM (°C) | D½ (M urea) | m-value (kcal mol−1 M−1) | ΔGH2O (kcal mol−1) | ku (s−1) | t½ (min) | |
|---|---|---|---|---|---|---|---|
| w.t. (Cys/Cys) | 21,000 | 70 | 4.6 | 2.1 | 16 | 5.1 × 10−6 | 2300 |
| Ala/Val | 20,000 | 74 | 4.1 | 1.3 | 11 | 7.3 × 10−4 | 16 |
| Ala/Ala | 20,000 | 56 | 2.3 | 0.9 | 7.7 | n.d. | n.d. |
Equilibrium parameters were determined from 2 to 3 trials. Unfolding rates were determined from 2 or 3 independent trials at 4–7 different urea concentrations (see Supporting Information).
a
M.R.E., mean residue ellipticity at 222 nm and 25°C; n.d., not determined.