Table II.
Crystallization Conditions
| Protein buffer | [Protein] (mg/mL) | Crystallization conditions | Ratio of protein: precipitant in crystallization drop | Cryo-protectant | |
|---|---|---|---|---|---|
| PDLIM1 | 50 mM Hepes pH 7.4, 500 mM NaCl, 0.5 mM TCEP | 63 | 40% PEG 300, 0.2M calcium acetate, 0.1M cacodylate pH 6.5 | 1:2 | None |
| PDLIM2 | 50 mM Hepes pH 7.4, 500 mM NaCl, 0.5 mM TCEP | 12 | 30% mPEG 2000, 0.2M (NH4)2SO4, 0.1M acetate pH 4.6 | 1:2 | 30% Glycerol |
| PDLIM4 | 50 mM Hepes pH 7.4, 500 mM NaCl, 0.5 mM TCEP | 6 | 20% PEG 10000, 0.2M MgCl2, 0.1M Tris pH 8.5 | 2:1 | 15% PEG400 |
| PDLIM5 | 25 mM Hepes pH 7.4, 150 mM NaCl, 0.5 mM TCEP | 13 | 30% PEG 4000, 0.2M MgCl2, 0.1M Tris pH 8.5 | 2:1 | 20% Ethylene glycol |
| PDLIM7 | 50 mM Hepes pH 7.4, 500 mM NaCl, 0.5 mM TCEP | 38 | 20% PEG 4000, 0.1M Hepes pH 7.5, 10% isopropanol | 1:2 | 20% Ethylene glycol |
| GRASP | 50 mM Hepes pH 7.4, 500 mM NaCl, 0.5 mM TCEP | 50 | 1.26M NaH2PO4, 0.14M K2HPO4 | 1:1 | 30% Glycerol |
| MAST4 | 25 mM Hepes pH 7.5, 150 mM NaCl, 0.5 mM TCEP | 17 | 0.75M NaH2PO4, 0.91M K2HPO4 | 2:1 | 25% Ethylene glycol |