Table II.
Detergent Screening Analysis via Gaussian Peak Fitting
| Initial solubilization |
48-h post solubilization |
||||
|---|---|---|---|---|---|
| Protein | Detergent | Number of Gaussian functions required | R2 | Number of Gaussian functions required | R2 |
| ArsB | LDAO | 5 | 1.00 | — | — |
| OG | 1 | 0.98 | 2 | 0.96 | |
| DM | 1 | 0.99 | 1 | 0.99 | |
| DDMa | 1 | 1.00 | 1 | 0.98 | |
| DsdX | LDAO | 3 | 1.00 | — | — |
| OG | 5 | 0.97 | — | — | |
| DMa | 1 | 1.00 | 1 | 0.99 | |
| DDM | 1 | 1.00 | 3 | 0.98 | |
| GntPb | — | — | — | — | |
| GntU | LDAO | 3 | 1.00 | — | — |
| OG | 6 | 0.99 | — | — | |
| DMa | 1 | 1.00 | 1 | 1.00 | |
| DDM | 1 | 0.97 | 1 | 0.99 | |
| LldP | LDAO | 4 | 1.00 | — | — |
| OG | 2 | 0.95 | — | — | |
| DMa | 1 | 1.00 | 1 | 0.99 | |
| DDM | 1 | 1.00 | 3 | 0.99 | |
| Mtr | LDAO | 4 | 0.99 | — | — |
| OG | 1 | 0.99 | 3 | 0.96 | |
| DM | 1 | 0.99 | 1 | 0.99 | |
| DDMa | 1 | 1.00 | 1 | 0.99 | |
| SdaC | LDAO | 2 | 0.99 | — | — |
| OG | 1 | 0.99 | 2 | 0.98 | |
| DM | 1 | 0.99 | 1 | 0.99 | |
| DDMa | 1 | 1.00 | 1 | 0.98 | |
| TdcC | LDAO | 3 | 1.00 | — | — |
| OG | 2 | 0.98 | — | — | |
| DM | 1 | 1.00 | 1 | 0.99 | |
| DDMa | 1 | 1.00 | 1 | 0.99 | |
| TyrP | LDAO | 3 | 0.99 | — | — |
| OG | 1 | 1.00 | 2 | 0.98 | |
| DM | 1 | 0.99 | 1 | 1.00 | |
| DDMa | 1 | 1.00 | 1 | 0.99 | |
| ChaA | LDAO | 1 | 0.98 | 1 | 0.97 |
| OG | 1 | 0.98 | 1 | 0.97 | |
| DMa | 1 | 0.99 | 1c | 0.98 | |
| DDM | 1 | 0.99 | 1 | 0.96 | |
| YrbG | LDAO | 4 | 0.98 | — | — |
| OG | 3 | 0.93 | — | — | |
| DM | 3c | 0.99 | 2d | 0.99 | |
| DDM | 1 | 0.99 | 2d | 0.82 | |
R2 is an indicator of how well the calculated Gaussian curve fits the experimental data.
a
The optimal detergent selected for solubilization and purification.
b
Solubilized protein levels were lower than needed for detection.
c
Major peak has a shoulder, still reanalyzed peak fraction 48 h post solubilization.
d
Calculated Gaussian Fit using mAu(λ = 485 nm), rather than mV, due to low protein abundance.