Table III.
Analysis of Protein Yields
| Protein construct | Yield (mg protein per L culture) |
|---|---|
| ArsB-His8 | 0.1 |
| ArsB-HRV3C | 1.0 (29%) |
| ArsB-HRV3C-GpA-GFP-His8 | 3.5 |
| DsdX-His8 | 1.7 |
| DsdX-HRV3C | 0 (0.0%) |
| DsdX-HRV3C-GpA-GFP-His8 | 2.9 |
| GntU-His8 | 0.1 |
| GntU-HRV3C | 1.9 (30%) |
| GntU-HRV3C-GpA-GFP-His8 | 6.4 |
| LldP-His8 | 0.2 |
| LldP-HRV3C | 3.5 (31%) |
| LldP-HRV3C-GpA-GFP-His8 | 11.4 |
| SdaC-His8 | 0.1 |
| SdaC-HRV3C | 1.3 (21%) |
| SdaC-HRV3C-GpA-GFP-His8 | 6.2 |
| TyrP-His8 | 0.8 |
| TyrP-HRV3C | 3.8 (39%) |
| TyrP-HRV3C-GpA-GFP-His8 | 9.7 |
Values in parentheses are the recovery rates after cleavage removal of the GpA-GFP fusion tag.