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. Author manuscript; available in PMC: 2010 May 11.
Published in final edited form as: J Immunol. 2008 Oct 1;181(7):4955–4964. doi: 10.4049/jimmunol.181.7.4955

Figure 5. Cytokine responses of lung T cells to PPD.

Figure 5

Mice were primed with BCG and boosted with Ad85A either intradermally (BA i.d) or intranasally (BA i.n.). Lung cells were isolated 4 weeks after the boost and stimulated with PPD for 6 hours. The frequencies of IFNγ, IL-2 and TNF producing cells were determined by flow cytometry on CD4 (A) or CD8 (C) gated cells and total numbers of cells per organ calculated. The number of cells expressing each of the seven possible combinations of the cytokines is shown only for CD4 (B) cells, but not for CD8 because the numbers are too low for reliable analysis. Results are expressed as the mean +/− SEM of three mice per group, representative of two independent experiments. * p< 0.05 compared to B and BA i.d.