Skip to main content
. Author manuscript; available in PMC: 2010 May 11.
Published in final edited form as: Mol Cell. 2008 Jul 25;31(2):244–254. doi: 10.1016/j.molcel.2008.06.004

Figure 6. DTT prevents the inhibition of the activity of the SMN complex by β-lapachone.

Figure 6

Cell extracts treated with 20 μM β-lapachone, or 20 μM β-lapachone together with 20 mM DTT, or DMSO control were analyzed by non-reducing Western blot. The relative levels of monomer SMN (“redSMN”) were calculated as the percentage of that in DMSO control and shown by the blue bar. Assembly activities of the SMN complex were measured by magnetic beads snRNP assembly assay using the same set of treated extracts and shown by the red bar. Error bars represent SDs from 3 independent experiments.