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. Author manuscript; available in PMC: 2010 May 11.
Published in final edited form as: Biochemistry. 2008 Jun 24;47(25):6571–6582. doi: 10.1021/bi800099d

Table 2.

Kinetic parameters for DR_0079a

Substrate Km,
mM		 Vmax,
µmol/min mg protein		 kcat,
sec−1 		kcat/Km,
mM−1sec−1
CDP 0.082 ± 0.011 5.18 ± 0.17 1.67 20.37
dCDP 0.092 ± 0.010 3.52 ± 0.11 1.12 12.17
TDP 0.105 ± 0.023 3.18 ± 0.27 1.03 9.81
UDP 0.137 ± 0.033 4.19 ± 0.42 1.35 9.85
GDP 0.092 ± 0.026 1.21 ± 0.10 0.39 4.24
dGDP 0.051 ± 0.010 1.55 ± 0.09 0.51 10.0
ADP 0.217 ± 0.033 0.93 ± 0.13 0.29 1.34
dADP 0.145 ± 0.028 1.61 ± 0.09 0.52 3.59
IDP 0.046 ± 0.010 0.21 ± 0.04 0.06 1.30
TTP 0.138 ± 0.030 2.13 ± 0.15 0.69 4.97
UTP 0.124 ± 0.030 1.65 ± 0.12 0.53 4.28
CTP 0.034 ± 0.007 1.82 ± 0.10 0.59 17.22
dCTP 0.023 ± 0.004 0.92 ± 0.08 0.30 12.83
ATP 0.317 ± 0.090 0.91 ± 0.08 0.29 0.92
dATP 0.062 ± 0.009 0.95 ± 0.07 0.31 4.92
GTP 0.394 ± 0.089 0.99 ± 0.13 0.32 0.81
dGTP 0.083 ± 0.011 1.25 ± 0.10 0.40 4.82
ITP 0.147 ± 0.027 0.55 ± 0.05 0.18 1.19
dITP 0.088 ± 0.008 0.68 ± 0.05 0.22 2.5
a

All substrate concentrations were between 0.01 and 4.0 mM. Kinetic parameters were determined by non-linear curve fitting using GraphPad Prism software (v 4.00 for Windows, San Diego, CA)