Fig. 4.

Effect of nebulized SCC1 and SCC8 on survival, clinical scores, and weight loss in a mouse model of Pseudomonas pneumonia. A) Treatment protocol. Mice received treatment with nebulized SCC1, SCC8 or water one hour after infection with P. aeruginosa PA M57-15, and again every 12 h for a total of 4 doses. Mice were evaluated on day 3 (72 h). B) Kaplan-Meier survival curves. The survival of mice treated with SCC1, SCC8 and water were 100%, 83% and 17%, respectively (SCC1 vs. water p = 0.0043, SCC8 vs. water p = 0.0096). C) Clinical scores. Clinical scores for each of the three treatment groups are displayed as mean ± SD. Numbers in parentheses indicate the number of surviving animals available for analysis, if less than the initial group size of 6. At the 24 hour time point, SCC1 vs. water and SCC8 vs. water both p < 0.001 by ANOVA. D) Weight loss. Displayed are the mean % weight changes from inoculation weight ± SD for SCC1, SCC8 and water-treated mice. The SCC1 and SCC8 treatment groups were compared at 48 and 72 hours to the single living water-treated animal by 1 sample t-test (48 h: SCC1 vs. water, p = 0.0395, SCC8 vs. water, p = 0.0212; 72 h: SCC1 vs. water, p = 0.0037, SCC8 vs. water, p = 0.0356).