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. Author manuscript; available in PMC: 2010 May 11.
Published in final edited form as: Analyst. 2009 May 15;134(8):1548–1553. doi: 10.1039/b904191e

Table 1.

Oligonucleotide sequences used. The underlined regions of the sequence indicate the “stem” which keeps the hairpin closed in the absence of target RNA. CAL indicates the position of a CAL Fluor Red 590 dye which is a TAMRA equivalent

Name Sequence 5′ → 3′
RSV probe [C6Thiol]TTTTTTTTTTCGACGAAAAATGGGGCAAATACGTCG[CAL]
FemA target ACGCTCACTATGAGTTAAAGCTTGCTGAAGGTTATGA
RSV target TTTTTTATTTGCCCCATTTTTTTTTT
RSV primer1 GCTCTTAGCAAAGTCAAGTTGAATGA
RSV primer2 TGCTCCGTTGGATGGTGTATT
GAPDH primer1 GGTGGTCTCCTCTGACTTC
GAPDH primer2 CTCTTCCTCTTGTGCTCTTG