Table 1.
Oligonucleotide sequences used. The underlined regions of the sequence indicate the “stem” which keeps the hairpin closed in the absence of target RNA. CAL indicates the position of a CAL Fluor Red 590 dye which is a TAMRA equivalent
Name | Sequence 5′ → 3′ |
---|---|
RSV probe | [C6Thiol]TTTTTTTTTTCGACGAAAAATGGGGCAAATACGTCG[CAL] |
FemA target | ACGCTCACTATGAGTTAAAGCTTGCTGAAGGTTATGA |
RSV target | TTTTTTATTTGCCCCATTTTTTTTTT |
RSV primer1 | GCTCTTAGCAAAGTCAAGTTGAATGA |
RSV primer2 | TGCTCCGTTGGATGGTGTATT |
GAPDH primer1 | GGTGGTCTCCTCTGACTTC |
GAPDH primer2 | CTCTTCCTCTTGTGCTCTTG |