. Author manuscript; available in PMC: 2011 May 1.

Published in final edited form as: Biophys Chem. 2010 Mar 3;148(1-3):104–111. doi: 10.1016/j.bpc.2010.02.016

TABLE 3.

Best-fit helico-elastic parameters under different electrophoresis conditions. All experiments were carried out in 0.8% agarose gels containing 0.5 µg mL⁻¹ chloroquine phosphate. The quantity n_DP designates the number of data points associated with the fit. All other quantities are as defined in the text.

Plasmid Series	n_DP	Buffer^*	P, bp	C, 10⁻¹⁹ erg·cm	h₀, bp· turn⁻¹	h_r	α
pCS2.Xd (2484 – 2516 bp)	52	TAE	155.2	3.007	10.44	0.9744	0.2117
″	″	TBE	144.8	2.564	10.49	0.9813	0.2315
pFS2.Xd (3412 – 3430 bp)	45	TBE	158.3	2.243	10.38	0.99	0.3053

TBE buffer: 50 mM Tris-borate, 1 mM Na2EDTA; pH 8.4

TAE buffer: 40 mM Tris-acetate, 1 mM Na2EDTA; pH 7.9