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. 2010 May 15;24(10):1035–1044. doi: 10.1101/gad.1907110

Figure 5.

Figure 5.

Enhancer properties of transiently acetylated regions occupied by GR and CEBPβ. ChIP at days 0, 0.25, 1, and 10 of 3T3-L1 differentiation for p300 (A) or MED1 (B). Data are represented as the mean of three biological replicates ± SEM. Comparison between day 0 and either day 0.25 or day 1 was used for significance calculation. (C) Validation of predicted enhancers by the luciferase reporter assay. Reporter activity was measured in transfected cells at days 0, 1, 2, and 4 of 3T3-L1 differentiation. Average fold activation relative to empty vector at day 0 and standard deviation from triplicate samples were plotted. Comparison between days 0 and 1 was used for significance calculation. (D) Pparγ2 gene expression at days 0 and 1 in cells treated with nontargeted control (−) or GR siRNAs. Standard deviation from triplicate samples was plotted. (*) P < 0.05; (**) P < 0.01.