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. 2010 May 15;24(10):986–991. doi: 10.1101/gad.579910

Figure 4.

Figure 4.

Characterization of endogenous siRNAs and detection of increased AtSN1, solo LTR, and IG1 abundance in jmj14. (A) Detection of siRNAs derived from AtSN1, LTR, 5S, tasiRNA 1511, and miRNAs 159 and 319 in JAP3, jmj14, and nrpd1 mutants. (B) Detection of AtSN1 transcripts in JAP, jmj14, and nrpd1 mutants by quantitative PCR. (C,D) Detection of solo LTR abundance (C) and IG1 abundance (D) by quantitative PCR. (E) Analysis of DNA methylation at AtSN1 by PCR. AluI digestion is sensitive to cytosine methylation. Reduction of methylation results in reduced levels of the PCR product. (F) Analysis of DNA methylation at solo LTR. MspI digestion is sensitive to cytosine methylation. Quantification of triplicate samples was performed, of which one representative sample is shown.