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Absolute number of LT-HSCs derived from original 100 LT-HSCs in the recipients 6 months after 4-Gy irradiation
| Input CD34−LKS | Output CD34−LKS/harvest | Output LKS/harvest | |
|---|---|---|---|
| Puma+/+ | |||
| M1 | 100 | 227 | 5982 |
| M2 | 1287 | 20 108 | |
| Mean | 757 | 13 000 | |
| Puma−/− | |||
| M1 | 100 | 5139 | 76 703 |
| M2 | 7147 | 23 822 | |
| M3 | 3669 | 40 314 | |
| Mean | 5320* | 46 900 |
The value of CD34−LKS/harvest was calculated using the total number of BMNCs per harvest multiplied by the percentage of CD34−LKS in BMNCs with flow cytometric analysis.
P < .05.
