Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 May 10;207(5):1015–1029. doi: 10.1084/jem.20090557

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Engel et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Vα14i NKT cells in CD8 transgenic mice. (A) Flow cytometric analysis of thymocytes from (top) WT mice, mice homozygous for the CD8 transgene (CD8 Tg/Tg; middle), and CD8 Tg hemizygous mice (CD8 Tg/+; bottom). The first column shows anti–TCR-β and αGalCer-CD1d tetramer staining, the second column shows Vα14i NKT cells, defined as gated in the left column, stained for CD4 and CD8α, the third column shows CD4 and CD8α staining of total thymocytes, and the fourth column shows the staining of Vα14i NKT thymocytes for NK1.1 and CD44, which was used to determine the percentages of cells within the stage 1 (CD44^low, NK1.1^low, bottom left box), stage 2 (CD44^high, NK1.1^low, top left box) and stage 3 (CD44^high, NK1.1^high, right box) Vα14i NKT developmental subsets. Data are representative of at least nine independent experiments. (B) Flow cytometric analysis of liver mononuclear cells (LMCs) obtained from the indicated mice stained with αGalCer-CD1d tetramers together with antibodies against TCR-β and CD8α. The left column shows staining with αGalCer-CD1d tetramers and anti–TCR-β to define the Vα14i NKT and conventional T subsets, with CD8α staining depicted for Vα14i NKT cells (middle column) and conventional T cells (right column). Data are representative of more than six independent experiments. (C) Scatter plot of the number of Vα14i NKT cells observed in thymus, spleen, and livers of CD8 Tg/+ and WT littermate mice. Matching symbol styles represent numbers determined from littermates on the same day. Paired Student's t test p-values are shown, with significant differences indicated by asterisks (**, 0.001 ≥ P ≤ 0.01; ***, P < 0.001). Error bars show SD and horizontal bars show the arithmetic mean of the samples in each column.