Figure 3.

Response of CD8 Tg Vα14i NKT cells to antigen in vivo. CD8 Tg/+ and WT littermate mice were injected i.p. with 2 µg αGalCer. Mice were killed 90 min later, and liver and spleen cell suspensions were prepared and stained with αGalCer-CD1d tetramers and additional antibodies to define Vα14i NKT cells. Depicted is the intracellular staining of Vα14i NKT cells from αGalCer-challenged mice with anti-IFNγ (A) and anti–IL-4 (B) mAbs as compared with the staining of Vα14i NKT cells from mock-challenged mice. Data are representative of four independent experiments.