Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 May 3;189(3):589–600. doi: 10.1083/jcb.201003007

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Mendez et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — NCad regulates the long-term stabilization of newly formed spines. (A) Stability over the next 2 d of new spines formed during an interval of 5 h in EGFP (Ctrl)-, EGFP + NCad (NCad)–, and EGFP + Δ390-NCad (Δ390)–transfected cells. Data are mean ± SEM of the proportion of spines still present at the indicated times (n = 5–16; 23, 15, and 50 new spines analyzed; *, P < 0.05; **, P < 0.01; two-way ANOVA with Bonferroni posttest). (B) Expression of DsRed2-tagged PSD-95 in newly formed spines (plus signs) observed in EGFP + NCad– or EGFP + Δ390-NCad–transfected neurons. Note that the new spine illustrated in the NCad condition expresses a DsRed2–PSD-95 puncta, but not the one in Δ390-NCad (arrowhead). (C) Fraction of new spines formed in an interval of 24 h (new spines) and of spines older than 24 h (preexisting spines) that expressed DsRed2–PSD-95 puncta in the three conditions tested. Data are mean ± SEM of 4–5 experiments (26, 13, and 19 new spines analyzed and 140, 82, and 75 stable spines analyzed in five control, four NCad, and five Δ390-NCad experiments, respectively; *, P < 0.05; **, P < 0.01; ***, P < 0.001; two-way ANOVA with Bonferroni posttest). Bar, 1 µm.