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. 2010 May 3;189(3):589–600. doi: 10.1083/jcb.201003007

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© 2010 Mendez et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Knockdown of NCad interferes with plasticity-induced spine stabilization. (A) Illustration of dendritic segments of pyramidal neurons cotransfected with mRFP and either control shRNA (left) or shRNA against NCad (right). (B) Expression of an shRNA against NCad (shNCad; n = 7 cells) results in a decrease in spine head width when compared with expression of a control shRNA (shCtrl; n = 6 cells). (C) The stability of spines of cells transfected with shNCad (n = 4) was reduced as compared with cells transfected with shCtrl (n = 7; mean ± SEM; *, P < 0.05; two-way ANOVA with Bonferroni posttest). (D) LTP induces an increased stabilization of activated spines (Act.) versus nonactivated spines (Non-act.) in cells transfected with shCtrl. Data are mean ± SEM of three experiments (79 spines analyzed; ***, P < 0.001; two-way ANOVA with Bonferroni posttest). (E) In contrast, LTP did not promote a selective stabilization of activated spines versus nonactivated ones in cells transfected with shNCad (n = 4; 86 spines analyzed; mean ± SEM). Bars, 2 µm.