Figure 5.
Exogenous expression of Dock180 and ELMO1 promotes glioma cell migration and invasion. A, U87MG and U251MG cells were transiently transfected with the indicated plasmids. Total cell lysates were analyzed by immunoblotting for ELMO1 and Dock180 expression. B and C, U87MG and U251MG cells were transiently transfected with the indicated plasmids followed by cell migration assay (B) or cell invasion assay (C). D, GFP-expressing U87MG cells were transiently transfected with the indicated plasmids and analyzed by an ex vivo brain slice invasion assay. Columns, mean distance (μm) invaded in 48 h from four independent experiments done in five to seven replicates per pair (control transfected–cells versus ELMO1- and Dock180-transfected cells); bars, SE. *, P < 0.05, paired Student’s t test.