Table 5.
Sugar Composition and Potential Biological Implication for Five of the Seven Glycans That Were Selected by All 10 Peak Sets
| Permethylated molecular weight of glycan we selected |
Structures of the glycan† | IUPAC code | Accurate permethylated molecular weight |
Oligosaccaride molecular weight |
Glycan Database ID+ |
Potential glycoprotein associated |
|---|---|---|---|---|---|---|
| 1580 |
|
Man a1-3 (Man a1-3 (Man a1-6) Man a1-6) Man b1-4 GlcNAc b1-4 GlcNAc | 1579.7834 | 1235.1172 | carbNlink_2 0482_D000 |
beta-glucuronidase 35, cytochrome P450 36, apolipoprotein B-100 42, IgM 43, Interleukin 6 44 |
| 2040‡ |
|
GlcNAc b1-2 Man a1-3 (Gal bl- 4 GlcNAc b1-2 Man a1-6) Man b1- 4 GlcNAc b1-4 (Fuc a1-6) GlcNAc or GlcNAc b 1-4 Man a1-3 (Gal bl- 4 GlcNAc bl-2 Man a1-6) Man b1- 4 GlcNAc b1-4 (Fuc a1-6) GlcNAc |
2040.0254 | 1641.5072 | carbNlink_2 1240_D000 |
IgG38,39,45 |
| 2187‡ |
|
NeuAc a2-3 Gal b1-4 GlcNAc bl-2 Man a1-3 (Man a1-3 Man a1- 6) Man b1-4 GlcNAc b1-4 GlcNAc |
2186.0834 | 1729.55 | carbNlink_4 3948_D000 |
human chorionic gonadotrophin 37 |
| 2851 |
|
GlcNAc b1-4 (NeuAc a2-6 Gal b1- 4GlcNAc b1-2 Man a1-3) (Gal b1- 4 GlcNAc b1-2 Man a1-6) Man b1- 4 GlcNac b1-4 (Fuc a1-6) GlcNAc or GlcNAc b1-4 (Gal b1-4 GlcNAc b1- 2 Man a1-3) (NeuAc a2-6 Gal b1- 4 GlcNAc b1-2 Man a1-6) Man b1- GlcNAc4 GlcNAc b1-4 (Fuc a1-6) GlcNAc |
2850.4252 | 2282.09 | carbNlink_4 1422_D000 |
IgGl40,41 |
| 4502*‡ |
|
NeuAc a2-6=2%| NeuAc a2-6=1%| 2% 1% NeuAc a2-6 Gal b1- 4 GlcNac b1-2 (2% 1% NeuAc a2- 6 Gal b1-4 (Fuc a1-3) GlcNAc b1-4) Man a1-3/6 (2% 1% NeuAc a2-6 Gal b1-4 GlcNAc b1-2 Man a1-6/3) Man b1-4 GlcNAc b1-4 GlcNAC |
4500 | 3625.2761 | carbNlink_2 1379_P |
CD45 (PTPRC protein tyrosine phosphatase, receptor type, C)46 |
†
Symbol for representation of Sugar type ■ GlcNAc; ○ Man; ● Gal; △ Fuc; ◆ NeuAc.
*
Structural analysis of this glycan is underway. According to the molecular weight and isotopic distribution of this glycan, we found this possible match by searching through glycan database.
+
The information was obtained from glycan database Web site: http://www.functionalglycomics.org/glycomics/molecule/jsp/carbohydrate/carbMoleculeHome.jsp