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. 2010 Feb 16;298(5):E1027–E1035. doi: 10.1152/ajpendo.00642.2009

Fig. 3.

Fig. 3.

Fatty acid and Thap-mediated cell death in primary hepatocytes from wild-type (WT) and Chop-knockout (KO) mice. Primary hepatocytes were incubated in LG or control medium supplemented with Thap (450 nM), albumin-bound oleate at 200 μM (O200), or palmitate at 200 μM (P200) for 16 h. Measurements of cell integrity include DNA fragmentation (representative gel), ELISA-based cell death, caspase-3 activity, and MTT assay. Data in graphs are reported as the mean ± SD of triplicate samples from a total of 6 independent experiments. *Significantly (P < 0.05) different from WT.