Fig. 6.

JNK activity and cell integrity in primary hepatocytes. Primary hepatocytes were incubated in LG or control medium supplemented with Thap (450 nM), albumin-bound oleate at 300 μM (O300), or palmitate at 100 (P100), 200 (P200), or 300 μM (P300) in the absence or presence of chemical chaperones, TUDCA (200 μM), PBA (500 μM), or SP600125 (10 μM) for 16 h. A and B: ratio of phosphorylation of c-Jun to total JNK. C: ELISA-based cell death. D: caspase-3 activity. Data in graphs are reported as the mean ± SD of triplicate samples from a total of 6 independent experiments. *Significantly (P < 0.05) different from no addition or SP600125. Representative Western blots are provided in Supplemental Fig. S9.