Table 1.

Effects of Chop SiRNA on the time course of palmitate-mediated cell death in H4IIE liver cells

	6 h		9 h		12 h		16 h
Palm, μM	Ctl	siRNA	Ctl	siRNA	Ctl	siRNA	Ctl	siRNA
100	33 ± 4	31 ± 5	36 ± 5	39 ± 4	65 ± 7	67 ± 8	92 ± 9	90 ± 11
200	29 ± 4	30 ± 3	33 ± 5	32 ± 4	101 ± 10	109 ± 11	177 ± 12	180 ± 13
300	35 ± 3	40 ± 4	96 ± 9	105 ± 11	196 ± 17	187 ± 16	256 ± 19	267 ± 22
400	39 ± 5	33 ± 4	137 ± 16	94 ± 9*	276 ± 19	145 ± 16*	361 ± 29	334 ± 30
500	26 ± 4	28 ± 2	195 ± 20	107 ± 11*	363 ± 29	241 ± 27*	427 ± 31	411 ± 39

Values are reported as means ± SD of triplicate samples from 6 independent experiments and are in mU/10³ cells. Chop, CCAAT/enhancer-binding protein homologous protein; Palm, palmitate; Ctl, small interfering (si)RNA control; siRNA, Chop siRNA. Cell death was measured by ELISA, as described in materials and methods.

^*Significantly (P < 0.05) different from Ctl at corresponding time point.