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RT-PCR detection of acid-sensing ion channel (ASIC) mRNA expression in mouse cerebral vessels. A: ASIC1. B: ASIC2. C: ASIC3. Whole brain or cerebellum was used as a positive control where noted. The presence of reverse transcriptase is indicated (RT+). Samples without RT served as negative controls. Base pair ladder (Ldr) is shown at left.
