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Western blot detection of ASIC proteins in isolated cerebral vessels (CV). Representative blots for ASIC1a and ASIC1b (A), ASIC2a/b, ASIC2a, and ASIC2b (B), and ASIC3 (C) are shown. β-Actin was used as a loading control for ASIC3. +/− denotes presence/absence of antigen. Arrowheads in C identify bands that are diminished in corresponding antigen control. MW, molecular weight.
