Fig. 6.
Current-voltage (I-V) relation changed by extracellular acidosis. A: I-V curves in pH 7.4 and pH 6.0 were acquired using a voltage-ramp protocol. Exposure to pH 6.0 increased the slope of I-V curve, indicating an increase in conductance during acidosis. The whole cell reversal potential (when I = 0) is also shifted to the right toward equilibrium potential of Na+ (ENa). Inset A: voltage-ramp protocol and waveform used in this experiment to acquire I-V curve. B: the net effect of pH 6.0 on I-V curve is shown, which is obtained by deducting the curve at pH 7.4 from that at pH 6.0. C: statistic shows a significant shift of the whole cell reversal potential to the right (*P < 0.05). The average net reversal potential is slightly positive (n = 5).